Macrophage Inflammatory Protein 4 (CCL18) Rabbit Polyclonal Antibody
CAT#: PP1054P1
Macrophage Inflammatory Protein 4 (CCL18) rabbit polyclonal antibody, Aff - Purified
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Specifications
Product Data | |
Applications | ELISA, FN, WB |
Recommended Dilution | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-4 (100.0 ng/ml), a concentration of 8.0-10.0 µg/ml of this antibody is required. Indirect ELISA: To detect hMIP-4 by indirect ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hMIP-4. Sandwich ELISA: To detect hMIP-4 by Sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MIP-4 (cat.-No PP1054B1 or PP1054B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMIP-4. Western Blot: To detect hMIP-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
Reactivities | Human |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Highly pure (>98%) E.coli derived recombinant Human MIP-4 |
Specificity | Specific for Human Macrophage Inflammatory Protein-4 (MIP-4). |
Formulation | PBS, pH 7.2 without preservatives State: Aff - Purified State: Lyophilized (sterile filtered) purified Ig fraction |
Reconstitution Method | Restore in sterile water to a concentration of 0.1-1.0 mg/ml. |
Purification | Affinity Chromatography using an immobilized Human MIP-4 matrix |
Storage | Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing. |
Stability | Shelf life: One year from despatch. |
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Background | This chemotactic factor that attracts lymphocytes but not monocytes or granulocytes may be involved in B cell migration into B cell follicles in lymph nodes. It attracts naive T lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T cells, CD4+ and CD8+ T cells and thus may play a role in both humoral and cell-mediated immunity responses. It is expressed at high levels in lung, lymph nodes, placenta, and bone marrow, not expressed by peripheral blood monocytes, and a monocyte-to-macrophage differentiation is a prerequisite for expression. Specifically induced in macrophages by IL4, IL13, and IL10, it belongs to the intercrine beta (chemokine CC) family. |
Synonyms | C-C motif chemokine 18, AMAC1, DCCK1, MIP-4, PARC, Small-inducible cytokine A18, SCYA18 |
Note | Centrifuge vial prior to opening! |
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