PF4 Rabbit Polyclonal Antibody
Specifications
Product Data | |
Applications | ELISA, FN, IHC, WB |
Recommended Dilution | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hPF-4 (50 ng/ml), a concentration of 1.75-3.0 µg/ml of this antibody is required. Direct ELISA: To detect hPF-4 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/well of recombinant hPF-4. Sandwich ELISA: To detect hPF-4 by Sandwich ELISA (using 100µl/well antibody solution) a concentration of 0.5-2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human PF-4 (Cat.-No PP1063B) as a Detection antibody, allows the detection of 0.2-0.4 ng/well of recombinant hPF-4. Western Blot: To detect hPF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPF-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry: The recommended concentration is 1.0-2.0 µg/ml with an overnight incubation at 4°C. An alkaline phosphatase-labeled polymer detection system was used with a red chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. |
Reactivities | Human |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Highly pure (>98%) E.coli derived recombinant human Platelet Factor-4 (hPF-4) |
Specificity | This antibody reacts with Human Platelet Factor 4 (PF-4). |
Formulation | PBS, pH 7.2 without preservatives State: Aff - Purified State: Lyophilized (sterile filtered) purified Ig fraction |
Reconstitution Method | Restore in sterile water to a concentration of 0.1-1.0 mg/ml. |
Purification | Affinity Chromatography employing immobilized Human PF-4 matrix |
Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. |
Stability | Shelf life: one year from despatch. |
Database Link | |
Synonyms | PF-4, Iroplact, Oncostatin-A, CXCL4, SCYB4 |
Note | Centrifuge vial prior to opening. |
Reference Data |
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