CXCL11 Rabbit Polyclonal Antibody

CAT#: PP1091B1

CXCL11 rabbit polyclonal antibody, Biotin

Product Images

Sandwich ELISA using PP1091B antibody

• 

  Direct ELISA using PP1091B antibody

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Direct ELISA: To detect Human I-TAC by Direct ELISA (using 100 µl/well antibody solution) a concentration of ~1.0 µg/ml of this antibody is required. This Biotin conjugated antibody allows the detection of at least 2000-4000 pg/ml of recombinant Human I-TAC.
  Sandwich ELISA: To detect Human I-TAC by Sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.25-1.0 µg/ml of this antibody is required. This Biotin conjugated antibody in conjuction with Purified anti-Human I-TAC (Cat.-No PP1091P) as a Capture antibody, allows the detection at least 2000-4000 pg/ml of recombinant Human I-TAC.
  Western Blot: To detect Human I-TAC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human I-TAC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) E.coli-derived 8.3 kDa recombinant Human I-TAC.
• Specificity: Recogbizes Human I-TAC.
  Other species not tested.
• Formulation: PBS, pH 7.2 without preservatives
  Label: Biotin
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 6373 Human
• Background: This member of the the intercrine alpha (chemokine CxC) family is chemotactive for interleukin-activated T cells but not unstimulated T cells, neutrophils or monocytes. It induces calcium release in activated T cells and binds to CXCR3. It may play an important role in CNS diseases which involve T cell recruitment and in skin immune responses. It is expressed at high levels in peripheral blood leukocytes, pancreas and liver astrocytes and at moderate levels in thymus, spleen and lung. It is induced by interferon gamma and interferon beta.
• Synonyms: C-X-C motif chemokine 11, Small-inducible cytokine B11, H174, Beta-R1, IP-9, I-TAC, SCYB11, SCYB9B
• Note: Centrifuge vial prior to opening!