FGF10 Goat Polyclonal Antibody

CAT#: PP1119P2

FGF10 goat polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, FN, WB
• Recommended Dilution: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hFGF-10 (1.5 ng/ml), a concentration of 0.02-0.035 µg/ml of this antibody is required.
  Indirect ELISA: To detect hFGF-10 by indirect ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hFGF-10.
  Sandwich ELISA: To detect hFGF-10 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human FGF-10 (PP1119B1 or PP1119B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hFGF-10.
  Western Blot: To detect hFGF-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
  Immunohistochemistry on frozen or paraffin sections: This antibody should be used at 0.5-2.0 µg/ml depending on dection system.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: E.coli derived 19.3 kDa Recombinant human FGF-10 (hFGF-10)
• Specificity: Fibroblast Growth Factor 10 (FGF-10)
• Formulation: PBS, pH 7.2 without preservatives.
  State: Aff - Purified
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity chromatography.
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 2255 Human
• Background: FGF10 is a heparin binding growth factor that stimulates the proliferation and activation of cells that express FGF receptors. FGF10 is mostly related to FGF-7/KGF and is expressed during development and preferentially in adult lungs.
• Synonyms: Fibroblast growth factor 10, FGF-10, Keratinocyte growth factor 2, KGF2
• Note: Centrifuge vial prior to opening!