BMP2 Rabbit Polyclonal Antibody

CAT#: PP1219B1

BMP2 rabbit polyclonal antibody, Biotin

Product Images

Direct ELISA using BMP2 Antibody Cat.-No PP1219B

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  Sandwich ELISA using BMP2 Antibody Cat.-No PP1219B

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Direct ELISA: To detect Human BMP-2 by Direct ELISA (using 100 µl/well antibody solution) a concentration of ~1.0 µg/ml of this antibody is required. This antibody in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant Human BMP-2.
  Sandwich ELISA: To detect Human BMP-2 by Sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.25-1.0 µg/ml of this antibody is required. This antibody in conjunction with Affinity purified anti-Human BMP2 (Cat.-No PP1219P1 or PP1219P2) as a Capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human BMP-2.
  Western Blot: To detect Human BMP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human BMP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) E.coli derived recombinant Human BMP-2.
• Specificity: The antibody recognizes Human BMP2. Other species not tested.
• Formulation: PBS, pH 7.2 without preservatives
  Label: Biotin
  State: Lyophilized (sterile filtered) purified Ig fraction
  Label: conjugated
• Reconstitution Method: Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography
• Conjugation: Biotin
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Database Link:
  Entrez Gene 650 Human
• Background: BMP2 belongs to the transforming growth factor beta (TGFB) superfamily of secreted growth factors. It is a disulfide linked homodimer and induces bone and cartilage formation. In addition to its osteogenic activity, BMP2 plays an important role in cardiac morphogenesis and is expressed in a variety of tissues including lung, spleen, brain, liver, prostate ovary and small intestine. The functional form of BMP2 is a 26 kDa protein composed of two identical 114 amino acid polypeptide chains linked by a single disulfide bond. BMPs control fundamental events in early embryonic development, organogenesis and adult tissue homeostasis.
• Synonyms: Bone morphogenetic protein 2, BMP-2, BMP-2A