Collagen II (COL2A1) Rabbit Polyclonal Antibody

CAT#: R1039B

Collagen II (COL2A1) rabbit polyclonal antibody, Biotin


USD 540.00

3 Days*

Size
    • 100 ug

Product Images

Specifications

Product Data
Applications ELISA, IHC, IP, WB
Recommended Dilution Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for Western blotting for highly sensitive qualitative analysis.
Recommended Dilutions:
ELISA: 1/10,000-1/50,000.
Western blot: 1/5,000-1/10,000.
Immunoprecipitation: 1/100.
Immunohistochemistry: 1/200-1/1,000.
Reactivities Bovine, Human, Mouse, Rat, Sheep
Host Rabbit
Isotype IgG
Clonality Polyclonal
Immunogen Collagen type II purified from adult Human knee and Bovine nasal cartilage.
For immunization collagens extensively purified by limited pepsin digestion and selective salt precipitation were used. This preparation results in a native conformation of the protein.
Specificity Crossreactivity < 1% to other collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has negligible cross-reactivity with Type I, III, IV, V or VI collagens.
Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Formulation 0.1M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA, pH 8.0
Label: Biotin
State: Lyophilized purified IgG fraction
Stabilizer: 10 mg/ml BSA (IgG and Protease free)
Preservative: 0.01% (w/v) Sodium Azide
Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Molar radio: 10-20 BAC molecules per Rabbit IgG molecule
Reconstitution Method Restore with 0.1 ml deionized water (or equivalent)
Concentration lot specific
Purification Immunoaffinity Chromatography using immobilised antigens followed by extensive cross-adsorbtion against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
Conjugation Biotin
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.
Synonyms COL2A1, Alpha-1 type II collagen
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.