Collagen III (COL3A1) Rabbit Polyclonal Antibody

CAT#: R1040B

Collagen III (COL3A1) rabbit polyclonal antibody, Biotin


USD 540.00

3 Days*

Size
    • 100 ug

Product Images

Specifications

Product Data
Applications ELISA, IHC, IP, WB
Recommended Dilution Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis.
Recommended Dilutions:
ELISA: 1/10,000-1/50,000.
Western blot: 1/1,000-1/5,000.
Immunoprecipitation: 1/100.
Immunohistochemistry: 1/200 -1/1,000.
Reactivities Bovine, Human
Host Rabbit
Isotype IgG
Clonality Polyclonal
Immunogen Collagen Type III from Human and Bovine placenta.
Specificity This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
Typically less than 1% cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.
This antibody reacts with most mammalian Type III collagens and has negligible cross-reactivity with Type I, II, IV, V and VI collagens.
Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Formulation 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA, pH 8.0
Label: Biotin
State: Liquid (sterile filtered) purified Ig fraction
Stabilizer: 10 mg/ml BSA (Ig and Protease free)
Preservative: 0.01% (w/v) Sodium Azide
Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Concentration lot specific
Purification Immunoaffinity Chromatography
Conjugation Biotin
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with type I Collagen. Mutations in the gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.
Synonyms COL3A1
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.