D Amino Acid Oxidase (DAO) Sheep Polyclonal Antibody

CAT#: R1056

D Amino Acid Oxidase (DAO) sheep polyclonal antibody, Serum

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: ELISA: 1/5,000-1/20,000
  Western blot: 1/500-1/5,000
  Immunoprecipitation: 1/100
  This product has been assayed against 1.0 ug of D-amino-acid oxidase [pig kidney] in a standard sandwich ELISA using peroxidase conjugated affinity purified anti-sheep IgG [H&L] (goat) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/100,000 of the reconstitution concentration is suggested.
• Reactivities: Porcine
• Host: Sheep
• Clonality: Polyclonal
• Immunogen: D-amino-acid oxidase from porcine kidney
• Specificity: This antibody detects porcine D-amino-acid oxidase.
  Immunoelectrophoresis give a single precipitin arc against purified and partially purified D-amino-acid oxidase [pig kidney].
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2, with 0.01% Sodium azide as preservative.
  State: Serum
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore with 2.0 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Prepared from monospecific antiserum by a delipidation and defibrination.
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: D-amino-acid oxidase
• Background: This gene encodes the peroxisomal enzyme D-amino-acid oxidase. The enzyme is a flavoprotein which uses flavin adenine dinucleotide (FAD) as its prosthetic group. Its substrates include a wide variety of D-amino-acids, but it is inactive on the naturally occurring L amino acids. Its biological function is not known; it has been suggested that it is involved in acid base balance in the kidney or it could act as a detoxifying agent which removes D-amino-acids accumulated during aging, or it may be a fossil enzyme without a current function.
• Synonyms: DAO, DAMOX, DAAO, OXDA, DAO1