DAO Sheep Polyclonal Antibody
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Specifications
Product Data | |
Applications | ELISA, IP, WB |
Recommended Dilution | Western blot: 1/500-1/5,000. Immunoprecipitation: 1/100. ELISA: 1/5,000-1/20,000. This product has been assayed against 1.0 µg of D-amino-acid oxidase [pig kidney] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/12,000 to 1:60,000 of the reconstitution concentration is suggested. |
Reactivities | Porcine |
Host | Sheep |
Clonality | Polyclonal |
Immunogen | D-amino-acid oxidase from porcine kidney |
Specificity | This antibody detects porcine D-amino-acid oxidase. Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-sheep serum as well as purified and partially purified D-amino-acid oxidase [pig kidney]. |
Formulation | 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: HRP State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!) Label: Horseradish peroxidase |
Reconstitution Method | Restore with 0.1 ml of deionized water (or equivalent). |
Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
Purification | Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer |
Conjugation | HRP |
Gene Name | - |
Background | The DAO gene encodes the peroxisomal enzyme D amino acid oxidase. The enzyme is a flavoprotein which uses flavin adenine dinucleotide (FAD) as its prosthetic group. Its substrates include a wide variety of D amino acids, but it is inactive on the naturally occurring L amino acids. Its biological function is not known; it has been suggested that it is involved in acid base balance in the kidney or it could act as a detoxifying agent which removes D-amino acids accumulated during aging, or it may be a fossil enzyme without a current function. |
Synonyms | DAO, DAMOX, DAAO, OXDA, DAO1 |
Reference Data |
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