ansB Rabbit Polyclonal Antibody

CAT#: R1059BS

ansB rabbit polyclonal antibody, Biotin, Purified

Product Images

Western blot of biotin conjugated rabbit anti L-asparaginase antibody. Lane 1: L-asparaginase. Lane 2: none. Load: 100 ng per lane. Primary antibody: Biotin conjugated L-asparaginase antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking buffer for 30 min at RT. Predicted/observed size: 32 kDa for L-asparaginase. Other band (s): L-asparaginase splice variants and isoforms.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution:

  Western blot: 1/500.
  ELISA: 1/1,000.
  L-asparaginase antibody has been assayed against 1.0 µg of asparaginase in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/3,200 to 1/12,800 of the reconstitution concentration is suggested.

• Reactivities: Escherichia coli
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Asparaginase from E. coli
• Specificity: This antibody detects asparaginase [E. coli]. Cross reactivity against asparaginase from other tissues and species may occur but have not been specifically determined.
  Immunoelectrophoresis give a single precipitin arc against anti-biotin, anti-rabbit serum as well as purified and partially purified asparaginase [E. coli].
• Formulation: 0.01 M Potassium phosphate, 0.14 M Sodium Chloride, pH 7.4
  Label: Biotin
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
  Preservative: 0.01% (w/v) Sodium Azide
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: Biotin