Choline oxidase Goat Polyclonal Antibody

CAT#: R1074PS

Choline oxidase goat polyclonal antibody, Purified

Product Images

Goat anti Choline oxidase antibody was used to detect Choline 0xidase. Samples of ~1 and 0.25 g of purified Choline oxidase per lane were run by SDS-PAGE under reducing and non-reducing conditions and reduced samples of purified Choline oxidase contained 4

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: Western blot: 1/500-1/5,000.
  Immunoprecipitation: 1/100.
  ELISA: 1/5000-1:20,000.
  This product has been assayed against 1.0 µg of Choline oxidase [Alcaligenes spec.] in a standard sandwich ELISA using peroxidase conjugated affinity purified anti-Goat IgG and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/22,000 of the reconstitution concentration is suggested for this product.
• Reactivities: Alcaligenes
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Choline oxidase from Alcaligenes spec.
• Specificity: This product detects Choline oxidase from Alcaligenes spec. Cross reactivity against Choline Oxidase from other sources is unknown.
  Immunoelectrophoresis give a single precipitin arc against anti-goat serum as well as purified and partially purified Choline Oxidase [Alcaligenes spec.].
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  State: Purified
  State: Lyophilized purified Ig fraction
  Preservative: 0.01% (w/v) Sodium azide
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Background: Choline oxidase catalyzes the oxidation of choline and betaine aldehyde to betaine with concomitant consumption of oxygen and production of hydrogen peroxide.