CELA1 Rabbit Polyclonal Antibody

CAT#: R1078PS

CELA1 rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Suitable for immunoblotting (Western or dot blot), ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity.
  Recommended Dilutions:
  ELISA: 1/5,000-1/25,000
  Western blot: 1/500-1/3,000.
  This product has been assayed against 1.0 µg of elastase [porcine pancreas] in a standard sandwich ELISA using peroxidase conjugated affinity purified anti-rabbit IgG and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,400 of the reconstitution concentration is suggested for this product.
  
  
• Reactivities: Porcine
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Elastase from porcine pancreas
• Specificity: This antibody detects porcine elastase. Cross reactivity against elastase from other tissues and species has not been determined.
  Immunoelectrophoresis gives a single precipitin arc against anti-rabbit serum as well as purified and partially purified elastase [porcine pancreas].
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  State: Purified
  State: Lyophilized purified Ig fraction
  Preservative: 0.01% Sodium azide
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Database Link:
  Entrez Gene 396766 Pig
• Background: Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Elastase is a serine protease which exhibits endopeptidase activity on many proteins, its principal substrate, however, is elastin and it is unique among proteases in its ability to hydrolyse native elastin. It is present in the pancreas, leukocytes and serum. Elastase consists of a single polypeptide chain with a molecular weight of 29.5kDa. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Like most of the human elastases, elastase 2A is secreted from the pancreas as a zymogen. In other species, elastase 2A has been shown to preferentially cleave proteins after leucine, methionine, and phenylalanine residues. Elastase 1 is not a pancreatic enzyme: clinical literature that describes human elastase 1 activity in the pancreas is actually referring to elastase 2A.
• Synonyms: ELA1, CELA1