GOT1 Sheep Polyclonal Antibody

CAT#: R1087PS

GOT1 sheep polyclonal antibody, Purified


USD 530.00

3 Days*

Size
    • 100 ug

Product Images

Specifications

Product Data
Applications ELISA, WB
Recommended Dilution Western blot: 1/500-1/3,000.
ELISA: 1/5,000-1/25,000.
This antibody has been assayed against 1.0 µg of aspartate transaminase [Porcine heart] in a standard sandwich ELISA using peroxidase conjugated affinity purified goat anti-sheep IgG [H&L] (R1364HRP) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/7,000 to 1/26,000 of the reconstitution concentration is suggested.
Reactivities Porcine
Host Sheep
Clonality Polyclonal
Immunogen Aspartate aminotransferase / GOT1 from porcine heart
Specificity This antibody detects porcine GOT1. 
Immunoelectrophoresis gives a single precipitin arc against anti-sheep serum as well as purified and partially purified aspartate transaminase / GOT1 [porcine heart].
Formulation 0.02M Potassium phosphate, 0.15M Sodium chloride, pH 7.2
State: Purified
State: Lyophilized purified Ig fraction
Preservative: 0.01% Sodium azide
Reconstitution Method Restore with 0.1 ml of deionized water (or equivalent).
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Purification Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Gene Name -
Background Aspartate aminotransferase (Glutamate oxaloacetate transaminase / GOT1) is a ubiquitous pyridoxal phosphate-dependent enzyme which exists in both mitochondrial and cytosolic forms. The enzyme plays an important role in amino acid metabolism and in the urea and tricarboxylic acid cycles. The 2 isoenzymes are homodimeric. In liver about 80% of the enzyme activity is mitochondrial in origin, whereas in serum the enzyme activity is largely cytosolic. Although the mitochondrial and soluble forms of GOT are coded by different chromosomes, the 2 show close homology in amino acid sequence and were presumably derived from a common ancestral gene.
Serum GOT (with SGPT) levels are usually elevated in states of hepatocellular injury (injury to the liver cells), the highest levels are associated with hepatitis of a viral origin. High levels are also found after myocardial infarction, when SGPT levels are lower.
Synonyms Aspartate aminotransferase, Transaminase A
Note Aspartate aminotransferase (AST) is also referred to as glutamic oxaloacetic transaminase (GOT).
Conjugates available. Please ask for details.
Reference Data

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