Luciferase (Photinus pyralis) Goat Polyclonal Antibody

CAT#: R1096

Luciferase (Photinus pyralis) goat polyclonal antibody, Serum

Specifications

Product Data

• Applications: ELISA, IHC, IP, WB
• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation, Immunhistochemistry and most Immunological methods requiring high titer and specificity.
  Recommended Dilutions: This product has been assayed against 1.0 µg of Luciferase [Firefly] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20000 to 1:100000 of the reconstitution concentration is suggested for this product.
  For Western Blot, a recommended working dilution is 1:2000 - 1:10000.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Luciferase [Firefly].
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Luciferase [Firefly].
  Cross reactivity against Luciferase from other sources has not been determined
• Formulation: PBS, pH 7.2 containing 0.01% sodium azides as a preservative.
  State: Serum
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore with 2.0 ml of deionized water (or equivalent).
• Concentration: 95.0 mg/ml (by Refractometry)
• Purification: Prepared from monospecific antiserum by a delipidation and defibrination.
• Background: Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.