Papain Goat Polyclonal Antibody

CAT#: R1102HRPS

Papain goat polyclonal antibody, HRP, Purified


USD 540.00

3 Days*

Size
    • 100 ug

Product Images

Specifications

Product Data
Applications ELISA, IHC, WB
Recommended Dilution ELISA: 1/1,000-1/5,000.
Western blot: 1/500-1/2,500.
Immunochemistry on Paraffin and Frozen sections: 1/200- 1/1,000.
Reactivities Carica papaya
Host Goat
Isotype IgG
Clonality Polyclonal
Immunogen Papain from Carica papaya
Specificity This antibody detects papaya Papain. Cross reactivity against Papain from other sources may occur but has not been specifically determined. Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-goat serum as well as purified and partially purified Papain [Carica papaya].
Formulation 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
Label: HRP
State: Purified
State: Lyophilized purified Ig fraction
Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!)
Label: Horseradish peroxidase
Reconstitution Method Restore with 0.1 ml of deionized water (or equivalent).
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Purification Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation HRP
Background Papain belongs to the peptidase C1 family and hydrolyses proteins with broad specificity for peptide bonds. The proteolysis preference of Papain is at amino acids bearing a large hydrophobic side chain at the P2 position and is inhibited when Val is at position P1. Papain may cause allergic reactions in humans.
Synonyms Papaya proteinase I, PPI, Allergen Car p 1
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.