Papain Goat Polyclonal Antibody

CAT#: R1102HRPS

Papain goat polyclonal antibody, HRP, Purified

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/1,000-1/5,000.
  Western blot: 1/500-1/2,500.
  Immunochemistry on Paraffin and Frozen sections: 1/200- 1/1,000.
• Reactivities: Carica papaya
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Papain from Carica papaya
• Specificity: This antibody detects papaya Papain. Cross reactivity against Papain from other sources may occur but has not been specifically determined. Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-goat serum as well as purified and partially purified Papain [Carica papaya].
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  Label: HRP
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
  Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!)
  Label: Horseradish peroxidase
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: HRP
• Background: Papain belongs to the peptidase C1 family and hydrolyses proteins with broad specificity for peptide bonds. The proteolysis preference of Papain is at amino acids bearing a large hydrophobic side chain at the P2 position and is inhibited when Val is at position P1. Papain may cause allergic reactions in humans.
• Synonyms: Papaya proteinase I, PPI, Allergen Car p 1