Penicillinase Rabbit Polyclonal Antibody
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Specifications
Product Data | |
Applications | ELISA, IP, WB |
Recommended Dilution | ELISA: 1/5,000-1/20,000. This product has been assayed against 1.0 µg of Penicillinase [E.clocae] in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/20,000 of the reconstitution concentration is suggested. Western blot: 1/500-1/5,000. Immunoprecipitation: 1/100. |
Reactivities | Enterobacter |
Host | Rabbit |
Isotype | IgG |
Clonality | Polyclonal |
Immunogen | Penicillinase from Enterobacter cloacae |
Specificity | This antibody detects Penicillinase [E. clocae]. Cross reactivity against Penicillinase from other tissues and species may occur but have not been specifically determined. Immunoelectrophoresis give a single precipitin arc against anti-biotin, anti-rabbit serum as well as purified and partially purified Penicillinase [E. clocae]. |
Formulation | 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: Biotin State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Sodium azide |
Reconstitution Method | Restore with 0.1 ml of deionized water (or equivalent). |
Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
Purification | Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer |
Conjugation | Biotin |
Reference Data |
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