Sarcosine oxidase Goat Polyclonal Antibody

CAT#: R1110PS

Sarcosine oxidase goat polyclonal antibody, Purified

Product Images

Goat anti Sarcosine oxidase antibody Cat.-No. R1110P was used to detect Sarcosine oxidase. Samples of ~1 and 0.25 g of purified Sarcosine oxidase per lane were run by SDS-PAGE under reducing and non-reducing conditions and reduced samples contained 4% BME

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: Western blot: 1/500-1/5,000.
  Immunoprecipitation: 1/100.
  ELISA: 1/5,000-1/20,000.
  This product has been assayed against 1.0 µg of microbial Sarcosine oxidase in a standard ELISA using peroxidase conjugated affinity purified anti-goat IgG and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/3,000 to 1/12,000 of the reconstitution concentration is suggested.
• Reactivities: Bacteria
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Sarcosine oxidase [Microbial]
• Specificity: This antibody detects microbial Sarcosine oxidase. Cross reactivity against Sarcosine oxidase from other sources is unknown.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum as well as purified and partially purified microbial Sarcosine oxidase.
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  State: Purified
  State: Lyophilized purified Ig fraction
  Preservative: 0.01% Sodium azide
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Synonyms: Peroxisomal sarcosine oxidase, PIPOX, LPIPOX, PSO, L-pipecolate oxidase, L-pipecolic acid oxidase