Meso_4146 Goat Polyclonal Antibody

CAT#: R1118PS

Meso_4146 goat polyclonal antibody, Purified

Product Images

Uricase antibody was used to detect purified uricase under reducing and non-reducing conditions. Samples of ~1 and 0.25 ug of protein per lane were run by SDS-PAGE and reduced samples of purified uricase contained 4% BME and were boiled for 5 minutes. Protein was transferred to nitrocellulose and probed (lot 6732). Primary antibody was detected with Dylight 649 conjugated donkey anti-goat secondary antibody.

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  ELISA: 1/1000-1/2500;
  This product has been assayed against 1.0 µg of uricase [Bacillus species] in a standard sandwich ELISA using peroxidase conjugated affinity purified anti-goat IgG [H&L] (Goat) and ABTS as a substrate for 30 minutes at room temperature.
  Immunoprecipitation: 1/100.
  Conjugation and most immunological methods require high titer and specificity.
• Reactivities: Bacillus sp.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Uricase from bacillus species
• Specificity: This product detects uricase (Bacillus species). Cross reactivity against uricase from other sources is unknown.
  Immunoelectrophoresis gives a single precipitin arc against anti-goat serum as well as purified and partially purified uricase [Bacillus species].
  
  
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  State: Purified
  State: Lyophilized purified Ig fraction
  Preservative: 0.01% (w/v) Sodium azide
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Background: Uricase is the peroxisomal enzyme responsible for the degradation of urate into allantoin. Uricase is a protein of 300 to 400 amino acids, its sequence is well conserved. It is mainly localised in the liver. In legumes, 2 forms of uricase are found: in the roots, the tetrameric form; and, in the uninfected cells of root nodules, a monomeric form, which plays an important role in nitrogen-fixation.
• Synonyms: URIC, URO, UOX, EC 1.7.3.3, Urate oxidase