ycjM Goat Polyclonal Antibody

CAT#: R1150PS

ycjM goat polyclonal antibody, Purified

Product Images

Goat anti Sucrose phosphorylase antibody was used to detect purified Sucrose phosphorylase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified protein contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1/3000 dilution of primary antibody (on 4°C in blocking buffer). Detection shown was using Dylight 488 conjugated donkey anti goat secondary antibody. Images were collected using the BioRad VersaDoc System.

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: Western blot: 1/500-1/5,000.
  Immunoprecipitation: 1/100.
  ELISA: 1/5,000-1/20,000.
  This product has been assayed against 1.0 µg of Sucrose phosphorylase [E.coli] in a standard ELISA using peroxidase conjugated affinity purified anti-goat IgG and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/6,500 to 1/32,000 of the reconstitution concentration is suggested.
• Reactivities: Escherichia coli
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Sucrose phosphorylase from E.coli
• Specificity: This antibody detects Sucrose phosphorylase [E.coli]. Cross reactivity against Sucrose phosphorylase from other sources is unknown.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum as well as purified and partially purified Sucrose phosphorylase [E.coli].
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  State: Purified
  State: Lyophilized purified Ig fraction
  Preservative: 0.01% (w/v) Sodium azide
• Reconstitution Method: Restore with 0,1 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Synonyms: Sucrose glucosyltransferase (E. coli)