Maltose Phosphorylase Goat Polyclonal Antibody

CAT#: R1151

Maltose Phosphorylase goat polyclonal antibody, Serum

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and most immunological methods requiring high titer and specificity.
  Recommended Dilutions: This product has been assayed against 1.0 ug of Maltose Phosphorylase [E.coli] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.
• Reactivities: Escherichia coli
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Maltose Phosphorylase [E.coli].
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Maltose Phosphorylase [E.coli].
  Cross reactivity against Maltose Phosphorylase from other sources is unknown.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% sodium azide as preservative.
  State: Serum
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore with 2.0 ml of deionized water (or equivalent).
• Concentration: 90.0 mg/ml (by Refractometry)
• Purification: Prepared from monospecific antiserum by a delipidation and defibrination.
• Background: Maltose phosphorylase is a dimeric enzyme that catalyzes the conversion of maltose and inorganic phosphate into beta D glucose 1 phosphate and glucose without requiring any cofactors, such as pyridoxal phosphate. The enzyme is part of operons that are involved in maltose/malto oligosaccharide metabolism.