Laminin Rabbit Polyclonal Antibody

CAT#: R1153B

Laminin rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, IHC, IP, WB
• Recommended Dilution: Anti-Laminin antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for Immunoprecipitation and for Western blotting for highly sensitive qualitative analysis.
  Recommended Dilutions: This product was assayed by immunoblot and found to be reactive against Laminin at a dilution of 1:5,000 to 1:10,000. This product was also assayed against 1.0 ug of Laminin in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:8,000 of the stock concentration is suggested for this product. For Immunohistochemistry on paraffin embedded tissue dilute the product 1:50 to 1:200.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced against a mixture of synthetic peptides corresponding to regions of adherence on laminin.
• Specificity: This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against human serum proteins and collagen and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with human Laminin and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Fibronectin.
  Non-specific cross reaction of anti-Laminin antibodies with other human serum proteins or non-Laminin extracellular matrix proteins is negligible.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% sodium azide as preservative and 10 mg/ml Bovine Serum Albumin (Ig and Protease free) as stabilizer.
  Label: Biotin
  State: Liquid (sterile filtered) purified Ig fraction.
  Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
  Molar radio: 10-20 BAC molecules per Rabbit IgG molecule.
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Conjugation: Biotin