Ferret IgG (gamma chain specific) Goat Polyclonal Antibody
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Specifications
Product Data | |
Recommended Dilution | This product is designed for Immunofluorescence Microscopy, fluorescence based plate assays (FLISA) and Fluorescent Western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Recommended Dilutions FLISA: 1/10,000-1/50,000. IF Microscopy: 1/1,000-1/5,000. Flow Cytometry: 1/500-1/2,500. |
Reactivities | Felinerret |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Ferret IgG γ gamma chain. |
Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Ferret IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Ferret IgG and Ferret Serum. No reaction was observed against Ferret IgA or Ferret IgM. Specificity was confirmed by ELISA at less than 1% cross reactivity against other Ferret heavy or light chain isotypes. |
Formulation | 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 with 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative. Label: FITC State: Lyophilized purified Ig fraction. Label: Fluorescein isothiocyanate (Molecular Weight 390 daltons) Absorption emission: 495 nm / 528 nm Molar radio: 4.0 moles FITC per mole of Goat IgG. |
Reconstitution Method | Restore with 1.0 ml of deionized water (or equivalent). |
Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
Purification | Immunoaffinity Chromatography. |
Conjugation | FITC |
Synonyms | Ferret Immunoglobulin G |
Reference Data |
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