Cyanine Dyes Sheep Polyclonal Antibody
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Specifications
Product Data | |
Applications | FC, IF |
Recommended Dilution | Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
Host | Sheep |
Clonality | Polyclonal |
Immunogen | A mixture of Cy3 and Cy5 conjugated KLH |
Specificity | This product was prepared from monospecific antiserum by Immunoaffinity chromatography using a cyanine BSA conjugate coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum and cyanine conjugated IgG. Reactivity was observed against Cy2, Cy3 and Cy5. Reactivity against other cyanine analog is unknown. |
Formulation | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide as preservative and 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer. Label: FITC State: Lyophilized purified Ig fraction Label: Fluorescein isothiocyanate (MW 390 daltons) Absorption emission: 495 nm / 528 nm Molar radio: 4.0 moles FITC per mole of Sheep IgG |
Reconstitution Method | Restore with 1.0 ml of deionized water (or equivalent). |
Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
Purification | Immunoaffinity chromatography |
Conjugation | FITC |
Reference Data |
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.