Aurora B (AURKB) Rabbit Polyclonal Antibody
Specifications
Product Data | |
Applications | Assay, ELISA, WB |
Recommended Dilution | This affinity purified antibody has been tested for use in ELISA (1:5,000-1:20,000) and by Western blot (1:500-1:2,000). Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 39 kDa in size corresponding to Aurora Kinase B by western blotting in the appropriate cell lysate or extract. Human brain tissue lysate or HeLa cell lysate can be used as a positive control. |
Reactivities | Human |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 227-238 of Human Aurora Kinase B protein. |
Specificity | This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human Aurora Kinase B protein. No reaction is expected against Aurora Kinase A. However, 100% sequence homology as indicated by BLAST analysis is on record for this protein from human, mouse, rat, cow, pig, dog and chimpanzee. Cross reactivity with Aurora Kinase B from other sources is not known. |
Formulation | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative. State: Aff - Purified State: Liquid (sterile filtered) purified Ig fraction. |
Concentration | 0.45 mg/ml (by UV absorbance at 280 nm) |
Purification | Immunoaffinity chromatography. |
Database Link | |
Background | Aurora Kinase B (also known as Aurora- and Ipl1-like midbody-associated protein 1, AIM-1, Aurora/IPL1-related kinase 2, Aurora-related kinase 2, STK-1, and Aurora-B) is a Ser/Thr protein kinase member of the Aurora subfamily that may be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Aurora Kinase B is localized to the midzone of central spindle in late anaphase and concentrated into the midbody in telophase and cytokinesis and is colocalized with gamma tubulin in the mid-body. High levels of Aurora B expression are seen in the thymus, although it is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Aurora B is expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase. |
Synonyms | Aurora-B, Aurora/IPL1-related kinase 2, STK-1, AURKB, AIK2, AIM1, ARK2, STK12 |
Reference Data |
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