Alanine aminotransferase 1 / ALT1 Sheep Polyclonal Antibody

CAT#: R1550HRPS

Alanine aminotransferase 1 / ALT1 sheep polyclonal antibody, HRP, Purified

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Western blot: 1/1,000-1/5,000.
  ELISA: 1/10,000-1/40,000.
  This antibody has been assayed against 1.0 µg of Alanine transaminase [pig heart] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000 to 1/3,000 of the reconstitution concentration is suggested.
• Reactivities: Porcine
• Host: Sheep
• Clonality: Polyclonal
• Immunogen: Alanine transaminase (ALT) from pig heart
• Specificity: This antibody detects porcine alanine transaminase. Cross reactivity against alanine transaminase from other sources is unknown.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-sheep serum as well as purified and partially purified alanine transaminase [pig heart].
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  Label: HRP
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/m BSA (immunoglobulin and protease free)
  Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!)
  Label: Horseradish peroxidase
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: HRP
• Background: Alanine transaminase (ALT) is also referred to as Glutamic pyruvic transaminase (GPT).
• Synonyms: GPT, AAT1, GPT1