Alanine aminotransferase 1 / ALT1 Sheep Polyclonal Antibody
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Specifications
Product Data | |
Applications | ELISA, WB |
Recommended Dilution | Western blot: 1/500-1/3000. ELISA: 1/5000-1/25000. This antibody has been assayed against 1.0 µg of Alanine transaminase [pig heart] in a standard sandwich ELISA using peroxidase conjugated affinity purified anti-sheep IgG [H&L] (goat) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/2,000 to 1/8,000 of the reconstitution concentration is suggested. |
Reactivities | Porcine |
Host | Sheep |
Clonality | Polyclonal |
Immunogen | Alanine transaminase (ALT) from pig heart |
Specificity | This antibody detects porcine alanine transaminase. Cross reactivity against alanine transaminase from other sources is unknown. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-sheep serum as well as purified and partially purified alanine transaminase [pig heart]. |
Formulation | 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 State: Purified State: Lyophilized purified Ig fraction Preservative: 0.01% (w/v) Sodium azide |
Reconstitution Method | Restore with 0.1 ml of deionized water (or equivalent). |
Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
Purification | Multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer |
Background | Alanine transaminase (ALT) is also referred to as Glutamic pyruvic transaminase (GPT). |
Synonyms | GPT, AAT1, GPT1 |
Note | Conjugates available. Please ask for details. |
Reference Data |
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