Alanine aminotransferase 1 / ALT1 Sheep Polyclonal Antibody

CAT#: R1550PS

Alanine aminotransferase 1 / ALT1 sheep polyclonal antibody, Purified


USD 530.00

3 Days*

Size
    • 100 ug

Product Images

Specifications

Product Data
Applications ELISA, WB
Recommended Dilution Western blot: 1/500-1/3000.
ELISA: 1/5000-1/25000.
This antibody has been assayed against 1.0 µg of Alanine transaminase [pig heart] in a standard sandwich ELISA using peroxidase conjugated affinity purified anti-sheep IgG [H&L] (goat) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/2,000 to 1/8,000 of the reconstitution concentration is suggested.
Reactivities Porcine
Host Sheep
Clonality Polyclonal
Immunogen Alanine transaminase (ALT) from pig heart
Specificity This antibody detects porcine alanine transaminase. Cross reactivity against alanine transaminase from other sources is unknown.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-sheep serum as well as purified and partially purified alanine transaminase [pig heart].
Formulation 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
State: Purified
State: Lyophilized purified Ig fraction
Preservative: 0.01% (w/v) Sodium azide
Reconstitution Method Restore with 0.1 ml of deionized water (or equivalent).
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Purification Multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Background Alanine transaminase (ALT) is also referred to as Glutamic pyruvic transaminase (GPT).
Synonyms GPT, AAT1, GPT1
Note Conjugates available. Please ask for details.
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.