CD45 / LCA Rat Monoclonal Antibody [Clone ID: YW 62.3]

CAT#: SM032FS

CD45 / LCA rat monoclonal antibody, clone YW 62.3, FITC

Specifications

Product Data

• Clone Name: YW 62.3
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of neat antibody to label 10e6 cells. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Mouse spleen cells. Spleen cells from immunised DA rat were fused with cells of the Y3/Ag1.2.3 myeloma cell line.
• Specificity: This antibody recognises the CD45 cell surface antigen, a 180-220kD glycoprotein expressed by all leucocytes.
• Formulation: PBS containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.
  Label: FITC
  State: Liquid purified IgG fraction.
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.1 mg/ml
• Purification: Affinity Chromatography on Protein G.
• Conjugation: FITC
• Background: CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200