CD45 / LCA Rat Monoclonal Antibody [Clone ID: IBL3/16]

CAT#: SM035P

CD45 / LCA rat monoclonal antibody, clone IBL3/16, Purified

Specifications

Product Data

• Clone Name: IBL3/16
• Applications: FC, IF, IHC, IP
• Recommended Dilution: Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 10⁶ cells in 100 µl.
  Immunofluorescence.
  Immunoprecipitation.
  Immunohistochemistry on Frozen Sections: 1/50-1/100.
  The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections.
  Not suitable for Paraffin, Resin Sections and Western blotting.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified B cells from mouse lymph nodes.
• Specificity: Recognizes Mouse CD45.
  It Enhances the MHC Class II induced cluster formation of mouse B cells.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purified IgG fraction from Tissue Culture Supernatant
  Stabilizer: 0.1% BSA
  Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purification: Affinity Chromatography Protein G
• Background: CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200