MHC Class II RT1Bu Mouse Monoclonal Antibody [Clone ID: OX-3]

CAT#: SM082F

MHC Class II RT1Bu mouse monoclonal antibody, clone OX-3, FITC

Specifications

Product Data

• Clone Name: OX-3
• Applications: FC
• Recommended Dilution: Flow cytometry: Use 10 µl of neat antibody to label 10e6 cells in 100 µl.
• Reactivities: Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Rat thymocyte membrane glycoproteins. Spleen cells from immunised BALB/c mice were fused with cells from the NS1 mouse myeloma cell line.
• Specificity: This antibody recognises a polymorphic determinant of Ia antigen (RT1.Bu) present on Lewis, Wistar and AO strain rats but not BN, DA or PVG/c strains. This antibody is useful for distinguishing Ia positive cells from different rat strains, e.g. for recognising cells of donor origin in bone marrow reconstituted radiation chimaeras. MRC OX-3 cross reacts with mouse strains of MHC haplotypes b and s, and analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. This antibody recognises Ia antigens on B-cells, dendritic cells and certain epithelial cells.
• Formulation: PBS, pH7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
  Label: FITC
  State: Liquid purified IgG
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.1 mg/ml
• Conjugation: FITC