MHC Class II RT1B (monomorphic) Mouse Monoclonal Antibody [Clone ID: OX-6]

CAT#: SM084F

MHC Class II RT1B (monomorphic) mouse monoclonal antibody, clone OX-6, FITC

Product Images

Staining of rat spleen cells with Mouse Anti Rat RT1B (Class II Monomorphic): FITC

Specifications

Product Data

• Clone Name: OX-6
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of Neat-1/10 diluted antibody to label 10e6 cells in 100 µl.
  This product is routinely tested in Flow Cytometry on rat splenocytes.
• Reactivities: Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Rat thymocyte membrane glycoprotein. Spleen cells from immunised BALB/C mice were fused with cells fo the mouse NS1 myeloma cell line.
• Specificity: This antibody recognises a monomorphic determinant of the I-A antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells.
  This antibody cross reacts with certain Mouse strains of MHC haplotypes k and s. Analysis of recombinant mouse strains has mapped the OX6 determinant to the H2IA region. (1, 6)
  This clone does not react with the Rat BDIX strain due to a defect in RT1B expression.(6)
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer
  Label: FITC
  State: Liquid purified IgG fraction.
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.1 mg/ml
• Purification: Affinity Chromatography on Protein G.
• Conjugation: FITC
• Background: The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In rats, this complex is referred to as the RT1 region. In mice, this complex is referred to as the H2 region.
• Synonyms: HLA Class II, MHC Class 2 RT1B