CD14 Mouse Monoclonal Antibody [Clone ID: UCHM1]
Specifications
Product Data | |
Clone Name | UCHM1 |
Applications | FC |
Recommended Dilution | Flow Cytometry: Use 10 µl of Neat-1/10 diluted antibody to label 10e6 cells or 100 µl whole blood. |
Reactivities | Human, Monkey |
Host | Mouse |
Isotype | IgG2a |
Clonality | Monoclonal |
Immunogen | Human Thymocytes followed by peripheral blood mononuclear cells. Spleen cells from mice immunised BALB/c mice were fused with cells from the NS1-Ag4/1 mouse myeloma line. |
Specificity | This antibody recognises a cell surface antigen of 55 kD, known as CD14. Antibodies to the CD14 molecule are known to induce oxidative burst formation. In tonsil tissue sections UCHM1 gives positive staining reactions with monocytic cells, the interfollicular tissue macrophages seen under the capsule, and dendritic reticulum cells. Skin Langerhans cells are always negative. UCHM1 also reacts with Kupffer cells and sinus lining cells on the liver. |
Formulation | PBS containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer. Label: FITC State: Liquid purified IgG fraction. Label: Fluorescein Isothiocyanate Isomer 1 |
Concentration | lot specific |
Purification | Affinity Chromatography on Protein G |
Conjugation | FITC |
Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Gene Name | CD14 molecule |
Database Link | |
Background | The CD14 molecule is found predominantly on monocytes and macrophages in flow cytometry, it is less strongly expressed on granulocytes, and is absent from stem cells and myeloid cells of very early differentiation states. |
Synonyms | CD14 |
Reference Data |
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