CD28 Rat Monoclonal Antibody [Clone ID: YTH913.12]

CAT#: SM1087PS

CD28 rat monoclonal antibody, clone YTH913.12, Purified


USD 340.00

5 Days*

Size
    • 100 ug

Product Images

Specifications

Product Data
Clone Name YTH913.12
Applications FC, IHC
Recommended Dilution Flow Cytometry: Use 10 µl of 1/25-1/50 diluted antibody to label 10e6 cells or 100 µl whole blood).
Immunohistochemistry on Frozen Sections.
Reactivities Human
Host Rat
Isotype IgG2b
Clonality Monoclonal
Immunogen Human peripheral blood T-cells.
Specificity This antibody recognises the human CD28 cell surface antigen, a 44kD glycoprotein expressed by a subset of T lymphocytes.
The antibody enhances the MLR response.
It has been reported that this clone also recognises an epitope of CD28 expressed by NK cells, which is not recognised by clones of 9.3 or CD28.2.(2)
Formulation PBS, pH 7.4, with 0.09 % Sodium Azide as preservative.
State: Purified
State: Liquid purified IgG fraction.
Concentration lot specific
Purification Affinity chromatography on Protein A.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Should this product contain a precipitate we recommend microcentrifugation before use.
Stability Shelf life: one year from despatch.
Background Human CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximately 95% of CD4+ cells and 50% of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells. The first is mediated via the T cell receptor following its interaction with antigenic peptide MHC complexes, and the second is delivered by accessory or costimulating molecules through their counter receptors on T lymphocytes. CD28 bears structural homology to CTLA 4 which is expressed at very low levels on the surface of CD4+ and CD8+ peripheral blood cells only following activation. CD28 is the natural receptor for the B7/BB 1 ligand (CD80) a 55-60 kDa glycoprotein which is expressed on activated B lymphocytes, on dendritic cells and on interferon gamma treated monocytes. The binding of B7 1/BB 1 molecules to CD28 is involved in T lymphocyte activation and in the initiation and maintenance of chronic inflammation.
Synonyms TP44
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.