CD57 / HNK1 Mouse Monoclonal Antibody [Clone ID: TB01]

CAT#: SM1143PS

CD57 / HNK1 mouse monoclonal antibody, clone TB01, Purified

Product Images

Staining of human peripheral blood lymphocytes with Mouse Anti Human CD57 (SM1143P/PS/PT).

Specifications

Product Data

• Clone Name: TB01
• Applications: FC, IHC
• Recommended Dilution: Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 10e6 cells in 100 µl.
  Immunohistochemistry on Frozen Sections (1/10-1/100).
  Immunohistochemistry on Paraffin Sections (1/10-1/50): This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections but sodium citrate buffer may enhance staining.
  Recommended Positive Control: Human tonsil.
• Reactivities: Human
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: Human neuroblastoma cells.
  Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3.X63 Ag8.653 myeloma cell line.
• Specificity: This antibody recognises the human CD57 molecule, expressed by NK cells and a subset of T cells.
• Formulation: PBS, pH7.4 containing 0.09% Sodium Azide as preservative.
  State: Purified
  State: Liquid purified IgM fraction.
• Concentration: 1.0 mg/ml
• Purification: Ion Exchange Chromatography.
• Background: CD57, also known as HNK-1, NK-1, and Leu-7, is a 100-115 kD oligosaccharide antigenic determinant expressed on a variety of proteins, lipids, and chondroitin sulfate proteoglycans. CD57 is expressed on a subset of peripheral blood lymphocytes, including NK cells and CD8+ T cells and is also expressed on neural cells and striated muscle. CD57 is not expressed on red cells, granulocytes, monocytes, or platelets.While the function of CD57 is unknown, binding to L-selectin, P-selectin, and a fragment of laminin suggests that CD57 may be involved in cell-matrix interactions. CD57 is increased in some disease states associated with CD4/CD8 imbalances (AIDS, autoimmune disease, viral infections, and allograft transplants).
• Synonyms: HNK-1, LEU7, NK-1, NK-Cell marker, NK1