CD8A Rat Monoclonal Antibody [Clone ID: YTC 182.20]

CAT#: SM1746F

CD8A rat monoclonal antibody, clone YTC 182.20, FITC

Product Images

Staining of human peripheral blood lymphocytes with RAT ANTI HUMAN CD8:FITC.

Specifications

Product Data

• Clone Name: YTC 182.20
• Applications: FC
• Recommended Dilution: Flow cytometry: use 10 µl of neat antibody to label 10e6 cells or 100 µl whole blood.
• Reactivities: Human
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Mouse L cells transfected with human CD8 gene. Spleen cells from an immunised DA rat were fused with cells of the rat Y3/Ag.1.2.3 myeloma line.
• Specificity: This antibody recognises the CD8 cell surface antigen, a 30/32kD glycoprotein expressed by the cytotoxic/suppressor subset of T lymphocytes, and more weakly by NK cells.
• Formulation: PBS, pH7.2 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
  Label: FITC
  State: Liquid purified IgG
  Label: Fluorescein Isothiocyanate Isomer 1 (FI
• Concentration: lot specific
• Conjugation: FITC
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens CD8a molecule (CD8A), transcript variant 1
• Database Link:
  Entrez Gene 925 Human
• Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
• Synonyms: CD8 alpha chain, CD8A, MAL

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
• Protein Pathways: Antigen processing and presentation, Cell adhesion molecules (CAMs), Hematopoietic cell lineage, Primary immunodeficiency, T cell receptor signaling pathway