CD45 (PTPRC) (CD45RA) Mouse Monoclonal Antibody [Clone ID: F8-11-13]

CAT#: SM1796P

CD45 (PTPRC) (CD45RA) mouse monoclonal antibody, clone F8-11-13, Purified

Specifications

Product Data

• Clone Name: F8-11-13
• Applications: FC, IHC
• Recommended Dilution: Flow cytometry (Use 10 µl of 1/50 - 1/100 diluted antibody to label 10e6 cells in 100 µl).
  Immunohistochemistry on frozen and paraffin embedded sections (1/200; Does not require protein digestion pre-treatment nor antigen retrieval using heat treatment).
  Recommended Positive Control Tissue: Human tonsil.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human T lymphocytes
• Specificity: Recognises the human CD45RA cell surface antigen.
  CD45 is a complex molecule existing in a number of isoforms. Antibodies recognising a common epitope on all of these isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
  Clone F8-11-13 reacts with the high molecular weight form of the leucocyte common antigen (LCA) expressed by B lymphocytes and a subset of T lymphocytes.
  This product is routinely tested in flow cytometry on human peripheral blood lymphocytes.
• Formulation: PBS buffer with 0.09% sodium azide as preservative
  State: Purified
  State: Liquid
• Concentration: lot specific
• Purification: Protein G affinity purified immunoglobulin fraction
• Storage: Store the antibody at 2-8°C for one month or at -20°C for longer. This product should be stored undiluted. Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Database Link:
  Entrez Gene 5788 Human
• Background: CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200