CD45 (PTPRC) (CD45RA) Mouse Monoclonal Antibody [Clone ID: F8-11-13]

CAT#: SM1796PT

CD45 (PTPRC) (CD45RA) mouse monoclonal antibody, clone F8-11-13, Purified

Product Images

Staining of formalin fixed, paraffin embedded human tonsil with Mouse anti Human CD45RA.

Specifications

Product Data

• Clone Name: F8-11-13
• Applications: FC, IHC
• Recommended Dilution: Immunohistology on Paraffin (1/200).
  Immunohistology on Frozen.
  Flow Cytometry (1/50-1/100): Use 10 µl of the suggested working dilution to label 10e6 cells in 100 µl.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human T lymphocytes.
  Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
• Specificity: This antibody recognises the human CD45RA cell surface antigen.
  Clone F8-11-13 reacts with the high molecular weight form of the leucocyte common antigen (LCA) expressed by B lymphocytes and a subset of T lymphocytes.
• Formulation: PBS, pH 7.2 containing 0.09% Sodium Azide as preservative.
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G.
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 5788 Human
• Background: CD45 is a complex molecule existing in a number of isoforms. Antibodies recognising a common epitope on all of these isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200