CD146 (MCAM) Rat Monoclonal Antibody [Clone ID: OJ79c]

CAT#: SM1860PS

CD146 (MCAM) rat monoclonal antibody, clone OJ79c, Purified


USD 430.00

5 Days*

Size
    • 100 ug

Product Images

Specifications

Product Data
Clone Name OJ79c
Applications FC, IF, IHC, IP, WB
Recommended Dilution ELISA: Use BSA free antibody for coating. 
Flow Cytometry: 0.5-1 µg/106 cells. 
Immunofluorescence: 0.5-1 µg/ml.
Western Blot: 0.5-1 µg/ml. 
Immunoprecipitation: 1-2 μg/500 µg protein lysate.
Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml for 30 minutes at RT.
Positive Control: A-375, HUV-EC or HeLa Cells, Tonsil or Melanoma.
Reactivities Human
Host Rat
Isotype IgG1
Clonality Monoclonal
Immunogen Recombinant Human MUC18  protein.
Specificity This antibody recognises the 118kD cell surface glycoprotein CD146.
Cellular Localization: Cell surface.
Formulation 10mM PBS
State: Purified
State: Liquid purified IgG fraction from Bioreactor Concentrate
Stabilizer: 0.05% BSA
Preservative: 0.05% Sodium Azide
Concentration lot specific
Purification Protein A/G Chromatography
Storage Store undiluted at 2-8°C.
DO NOT FREEZE!
Stability Shelf life: one year from despatch.
Predicted Protein Size 130 kDa
Background The Human Mel-CAM gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC18 or CD146, that belongs to the immunoglobulin superfamily and functions as a Ca2+-independent cell adhesion molecule. Mel-CAM expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. Mel-CAM is found on 80% of advanced primary human melanomas and correlates well with development of metastatic disease.
Synonyms MCAM, MUC18, CD146
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.