Clec7a Rat Monoclonal Antibody [Clone ID: 2A11]

CAT#: SM2025LE

Clec7a rat monoclonal antibody, clone 2A11, Low Endotoxin


USD 940.00

5 Days*

Size
    • 500 ug

Product Images

Specifications

Product Data
Clone Name 2A11
Applications FC, FN, IHC, IP
Recommended Dilution Functional Assays.
Immunoprecipitation.
Flow Cytometry: Use 1/10-1/50 diluted antibody to label 106 cells in 100 µl.
Immunohistochemistry on Frozen Sections: The epitope recognised by 2A11 is sensitive to H202 treatment, therefore quenching with 0.3% H202 should be performed after incubation with the primary antibody.
Reactivities Mouse
Host Rat
Isotype IgG2b
Clonality Monoclonal
Immunogen Dectin-1 transfected NIH3T3 cells and recombinant soluble Dectin-1.
Spleen cells from immunised Fischer rats were fused with cells of the rat Y3 myeloma cell line.
Specificity This antibody clone 2A11 recognises beta-Glucan Receptor, also known as Dectin-1.
This antibody inhibits the binding of zymosan to macrophages via the beta-glucan receptor. The low endotoxin version (SM2025LE) is recommended for functional studies.
Formulation PBS, pH 7.4
State: Low Endotoxin
State: Liquid purified IgG fraction
Stabilizer: None
Preservative: None
Concentration 1.0 mg/ml
Purification Affinity Chromatography on Protein G
Background Dectin-1 is predominantly expressed by cells of the monocyte/macrophage and neutrophil lineages, but also at lower levels by dendritic cells and a subpopulation of T cells. As a major leucocyte receptor for beta-glucan this molecule may have a key role in the immunomodulatory effects of beta-glucans and in the host response to fungal pathogens. Recent work has shown that Dectin-1 may stimulate reactive oxygen production in macropahges via the protein tyrosine kinase known as Syk.
Synonyms Beta-glucan receptor, BGR, CLECSF12, DECTIN1
Note Endotoxin Level: Less than 0.01 EU/µg
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.