Clec7a Rat Monoclonal Antibody [Clone ID: 2A11]

CAT#: SM2025LE

Clec7a rat monoclonal antibody, clone 2A11, Low Endotoxin

Product Images

Staining of Mouse peripheral blood monocytes with Rat Anti Mouse Beta Glucan Receptor antibody

Specifications

Product Data

• Clone Name: 2A11
• Applications: FC, FN, IHC, IP
• Recommended Dilution: Functional Assays.
  Immunoprecipitation.
  Flow Cytometry: Use 1/10-1/50 diluted antibody to label 10⁶ cells in 100 µl.
  Immunohistochemistry on Frozen Sections: The epitope recognised by 2A11 is sensitive to H₂0₂ treatment, therefore quenching with 0.3% H₂0₂ should be performed after incubation with the primary antibody.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Dectin-1 transfected NIH3T3 cells and recombinant soluble Dectin-1.
  Spleen cells from immunised Fischer rats were fused with cells of the rat Y3 myeloma cell line.
• Specificity: This antibody clone 2A11 recognises beta-Glucan Receptor, also known as Dectin-1.
  This antibody inhibits the binding of zymosan to macrophages via the beta-glucan receptor. The low endotoxin version (SM2025LE) is recommended for functional studies.
• Formulation: PBS, pH 7.4
  State: Low Endotoxin
  State: Liquid purified IgG fraction
  Stabilizer: None
  Preservative: None
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography on Protein G
• Database Link:
  Entrez Gene 56644 Mouse
• Background: Dectin-1 is predominantly expressed by cells of the monocyte/macrophage and neutrophil lineages, but also at lower levels by dendritic cells and a subpopulation of T cells. As a major leucocyte receptor for beta-glucan this molecule may have a key role in the immunomodulatory effects of beta-glucans and in the host response to fungal pathogens. Recent work has shown that Dectin-1 may stimulate reactive oxygen production in macropahges via the protein tyrosine kinase known as Syk.
• Synonyms: Beta-glucan receptor, BGR, CLECSF12, DECTIN1
• Note: Endotoxin Level: Less than 0.01 EU/µg