Clec7a Rat Monoclonal Antibody [Clone ID: 2A11]

CAT#: SM2025P

Clec7a rat monoclonal antibody, clone 2A11, Purified

Product Images

Staining of mouse peripheral blood monocytes with RAT ANTI MOUSE BETA GLUCAN RECEPTOR.

Specifications

Product Data

• Clone Name: 2A11
• Applications: FC, IHC, IP
• Recommended Dilution: ELISA.
  Immunohistochemistry on Frozen Sections: The epitope recognised by 2A11 is sensitive to H202 treatment, therefore quenching with 0.3% H202 should be performed after incubation with the primary antibody.
  Flow Cytometry: Use 10 µl of 1/10-1/50 diluted antibody to label 10e6 cells in 100 µl.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Dectin-1 transfected NIH3T3 cells and recombinant soluble Dectin-1.
  Spleen cells from immunised Fischer rats were fused with cells of the rat Y3 myeloma cell line.
• Specificity: This antibody recognises beta-glucan receptor, also known as Dectin-1. This antibody inhibits the binding of zymosan to macrophages via the beta-glucan receptor.
  The Low Endotoxin version (SM2025LE) is recommended for functional studies.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography on Protein G
• Database Link:
  Entrez Gene 56644 Mouse
• Background: Dectin-1 is predominantly expressed by cells of the monocyte/macrophage and neutrophil lineages, but also at lower levels by dendritic cells and a subpopulation of T cells. As a major leucocyte receptor for beta-glucan this molecule may have a key role in the immunomodulatory effects of beta-glucans and in the host response to fungal pathogens. Recent work has shown that Dectin-1 may stimulate reactive oxygen production in macropahges via the protein tyrosine kinase known as Syk.
• Synonyms: Beta-glucan receptor, BGR, CLECSF12, DECTIN1