3-mC Rabbit Polyclonal Antibody

CAT#: TA160024

Rabbit Polyclonal 3-methylcytosine (3-mC) Antibody

Product Images

Dot blot analysis using the antibody against 3-mC To demonstrate the specificity of the antibody against 3-mC, a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo' were spotted on the membrane. The antibody was diluted :400 in PBS-T containing 10 % skimmed milk and 1% BSA. Image shows a high specificity of the antibody for the oligonucleotide with the 3-methylcytosine modification.

Specifications

Product Data

• Applications: Dot
• Recommended Dilution: Dot blotting (1:400)
• Reactivities: Human, Mouse, broad range
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The immunogen for anti-3-mC antibody: 3-methylcytosine (3-mC) conjugated to BSA.
• Concentration: 1.80 ug/ul
• Purification: Protein G purified polyclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300.
• Background: 3-methylcytosine arises by erroneous methylation of DNA through spontaneous exposure to endogenous S-adenosyl methionine (SAM). Since the N3-position in cytosine is protected in double stranded DNA, this phenomenon mainly occurs in single stranded DNA. The resulting 3-mC is toxic and mutagenic and increased levels of 3-mC seem to impair cell proliferation. Therefore, it has to be repaired by the cell. This is established by oxidative demethylation, which is catalyzed by the AlkB protein.