Tfeb Goat Polyclonal Antibody

CAT#: TA302788

Goat Polyclonal Antibody against TFEB

Product Images

TA302788 (0.5ug/ml) staining of Human Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  TA302788 (0.5ug/ml) staining of HeLa (A) and (0.3ug/ml) Jurkat (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

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  TA302788 (2ug/ml) staining of paraffin embedded Human Kidney cancer. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1:64,000; WB: 0.3-1ug/ml
• Reactivities: Human
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence ASRIGLRMQLMRE-C, from the N Terminus of the protein sequence according to NP_009093.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: transcription factor EB
• Database Link:
  NP_035679
  Entrez Gene 7942 HumanEntrez Gene 316214 RatEntrez Gene 609046 DogEntrez Gene 21425 Mouse
  UniProt ID Q9R210
• Background: Probable transcription factor binds to the USF/MLTF site and probably recognizes E-box sequences in the heavy-chain immunoglobulin enhancer
• Synonyms: ALPHATFEB; BHLHE35; OTTHUMP00000043540; TCFEB
• Note: In paraffin embedded Human Kidney cancer shows strong nuclear staining of invading cells. This antibody was confirmed in ChIP on SK-MEL-5 cells (The result was obtained from a previous batch (different goat)).