IRAK (IRAK1) Rabbit Polyclonal Antibody

CAT#: TA305934

Rabbit Polyclonal IRAK Antibody

Product Images

Western blot analysis of IRAK in THP-1 (THP) and HeLa (HL) whole cell lysates with IRAK antibody at 1:2000

• 

  Immunohistochemistry of IRAK in HeLa cells with IRAK antibody at 10 ug/ml.

• 

  Immunocytochemistry of IRAK in HeLa cells with IRAK antibody at 10 ug/mL.

• 

  Immunofluorescence of IRAK in HeLa cells with IRAK antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 ug/mL, IP: 2 - 4 ug per sample, ICC: 10 ug/g/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: IRAK antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human IRAK.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Antibody is DEAE purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: interleukin 1 receptor associated kinase 1
• Database Link:
  NP_001020413
  Entrez Gene 16179 MouseEntrez Gene 363520 RatEntrez Gene 3654 Human
  UniProt ID P51617
• Background: Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFalpha and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently. IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response. IRAK mediates a signaling cascade leading to NF-kappaB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF).
• Synonyms: IRAK; pelle

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors
• Protein Pathways: Apoptosis, Neurotrophin signaling pathway, Toll-like receptor signaling pathway