STAT1 Rabbit Polyclonal Antibody

CAT#: TA305937

Rabbit Polyclonal STAT1 alpha Antibody

Product Images

Western blot analysis of STAT1 alpha in whole cell lysates from HeLa (H), Jurkat (J), A431 (A), K562 (K), and NIH3T3 (N) cells, with STAT1a antibody at 1ug/ml.

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  Immunohistochemistry of STAT1alpha in human colon tissue with STAT1alpha antibody at 2.5ug/ml.

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  Immunofluorescence of STAT1 alpha in human colon tissue with STAT1 alpha antibody at 20ug/ml.

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  Immunocytochemistry of STAT1 alpha in HeLa cells with STAT1 alpha antibody at 10ug/ml.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 0.5 - 1 ug/mL, IP: 4 ug/mL, ICC: 10 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: STAT1 alpha antibody was raised against a peptide corresponding to 38 amino acids near the carboxy terminus of human STAT1 alpha. The sequences differ from the murine corresponding sequences by four amino acids. The immunogen is located within the last 50 amino acids of STAT1 alpha.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Antibody is DEAE purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: signal transducer and activator of transcription 1
• Database Link:
  NP_009330
  Entrez Gene 20846 MouseEntrez Gene 25124 RatEntrez Gene 6772 Human
  UniProt ID P42224
• Background: STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a, Stat5b, and Stat6), Stat1, Stat3, Stat5alpha, and Stat5beta have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-alpha, IFN-beta, IFN-gamma and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). STAT1 has two forms, the 91 kDa STAT1alpha and the 84 kDa STAT1beta which are encoded by the same gene with splicing variant.
• Synonyms: CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Chemokine signaling pathway, Jak-STAT signaling pathway, Pancreatic cancer, Pathways in cancer, Toll-like receptor signaling pathway