FLASH (CASP8AP2) Rabbit Polyclonal Antibody

CAT#: TA306039

Rabbit Polyclonal FLASH Antibody

Product Images

Western blot analysis of FLASH in HeLa whole cell lysate with FLASH antibody at 0.5 ug/mL.

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  Immunocytochemistry of FLASH in HeLa cells with FLASH antibody at 10 ug/mL.

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  Immunofluorescence of FLASH in HeLa cells with FLASH antibody at 10 ug/mL.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 0.5 ug/mL, ICC: 10 ug/mL, IF: 10 ug/mL
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: FLASH antibody was raised against a synthetic peptide corresponding to amino acids near the carboxy-terminus of human FLASH which differ from those of mouse by one amino acid.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: caspase 8 associated protein 2
• Database Link:
  NP_001131139
  Entrez Gene 9994 Human
  UniProt ID Q9UKL3
• Background: A novel mammalian CED-4 homologous was recently identified and cloned in mouse (1) and human (2) and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8 (1). There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity (1,2). FLASH is widely expressed.
• Synonyms: CED-4; FLASH; RIP25

Reference Data

• Protein Families: Druggable Genome