BTK Rabbit Polyclonal Antibody

CAT#: TA306188

Rabbit Polyclonal BTK Antibody

Product Images

Western blot analysis of BTK in U937 cell lysate with BTK antibody at (A) 0.5, (B) 1, and (C) 2 ug/mL.

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  Immunocytochemistry of BTK in Daudi cells with BTK antibody at 10 ug/mL.

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  Immunofluorescence of BTK in Daudi cells with BTK antibody at 10 ug/mL.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 0.5 - 2 ug/mL, ICC: 10 ug/mL, IF: 10 ug/mL
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: BTK antibody was raised against a synthetic peptide corresponding to 16 amino acids near the amino-terminus of human BTK.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Bruton tyrosine kinase
• Database Link:
  NP_000052
  Entrez Gene 12229 MouseEntrez Gene 695 Human
  UniProt ID Q06187
• Background: Bruton’s tyrosine kinase (BTK) was initially identified as a member of the src family for protein-tyrosine kinases that was involved in X-linked agamma-globulinaemia (1), and has since been shown to involved in a number of signaling pathways in hemapoietic lineage (reviewed in 2). It has recently been shown to interact with members of the toll-like receptor (TLR) family such as TLR4, 6, 8, and 9 (3). The TLRs are critical molecules in both the innate and adaptive immunity and can recognize diverse microbial pathogens (reviewed in 4). BTK has also been shown to interact with key proteins involved in TLR4 signal transduction such as MyD88, TIRAP, and IRAK, but not TRAF-6, suggesting that BTK is involved in lipopolysaccharide signal transduction (3).
• Synonyms: AGMX1; AT; ATK; BPK; IMD1; PSCTK1; XLA

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: B cell receptor signaling pathway, Fc epsilon RI signaling pathway, Primary immunodeficiency