Tuberin (TSC2) Rabbit Polyclonal Antibody

CAT#: TA306220

Rabbit Polyclonal TSC2 Antibody

Product Images

Western blot analysis of TSC2 in L1210 cell lysate with TSC2 antibody at (A) 2 and (B) 4 ug/mL.

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  Immunocytochemistry of TSC2 in L1210 cells with TSC2 antibody at 10 ug/mL.

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  Immunofluorescence of TSC2 in L1210 cells with TSC2 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 2 - 4 ug/mL, ICC: 10 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: TSC2 (NT) was raised against a 14 amino acid peptide from near the amino terminus of human TSC2.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: tuberous sclerosis 2
• Database Link:
  NP_000539
  Entrez Gene 22084 MouseEntrez Gene 7249 Human
  UniProt ID P49815
• Background: Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors (reviewed in 1,2). TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high (3). Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro (4), suggesting that the TSC1/TSC2 complex decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing that overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth (4,5). At least three isoforms of TSC2 exist.
• Synonyms: LAM; PPP1R160; TSC4

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Insulin signaling pathway, mTOR signaling pathway, p53 signaling pathway