PKR (EIF2AK2) Rabbit Polyclonal Antibody

CAT#: TA306356

Rabbit Polyclonal PKR Antibody

Product Images

Western blot analysis of PKR in A431 whole cell lysate with PKR antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.

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  Immunohistochemistry of PKR in rat lung tissue with PKR antibody at 2.5 ug/ml.

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  Immunofluorescence of PKR in Rat Lung tissue with PKR antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 0.5 - 2 ug/mL, ICC: 2.5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: PKR antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human PKR.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: eukaryotic translation initiation factor 2 alpha kinase 2
• Database Link:
  NP_001129123
  Entrez Gene 19106 MouseEntrez Gene 54287 RatEntrez Gene 5610 Human
  UniProt ID P19525
• Background: The interferon-inducible, double-stranded RNA (dsRNA)-dependent protein kinase PKR is a member of the eukaryotic initiation factor-2 alpha (eIF2-alpha) kinase family, possessing serine-threonine kinase activity and two dsRNA-binding motifs that acts as part of the innate immune system. Upon binding dsRNA, PKR undergoes a conformational change leading to its activation and its phosphorylation of the translation factor eIF2, resulting in a general shutdown of protein synthesis and induction of apoptosis through upregulation of caspase-8 and capsase-9 activity in order to prevent the production of more viruses. To evade the antiviral effects of PKR, viruses have evolved multiple mechanisms, such as the inhibition of PKR by the non-structural protein (NS1) of the influenza virus. More recently, PKR has been implicated in several neurodegenerative diseases including Alzheimer, Huntington, and amyotrophic lateral sclerosis.
• Synonyms: EIF2AK1; PKR; PPP1R83; PRKR

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors