ARFBP1 (HUWE1) Rabbit Polyclonal Antibody

CAT#: TA306429

Rabbit Polyclonal ARF-BP1 Antibody

Product Images

Western blot analysis of ARF-BP1 in Daudi cell lysate with ARF-BP1 antibody at 1 ug/mL.

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  Immunocytochemistry of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 5 ug/mL.

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  Immunofluorescence of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1 ug/mL, ICC: 5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: ARF-BP1 antibody was raised against a 19 amino acid peptide from near the carboxy terminus of human ARF-BP1.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase
• Database Link:
  NP_113584
  Entrez Gene 59026 MouseEntrez Gene 501546 RatEntrez Gene 10075 Human
  UniProt ID Q7Z6Z7
• Background: The ARF tumor suppressor is a critical regulator of p53 stability. In addition to p53, ARF1 binds to other proteins such as MDM2 and ARF-BP1, a large protein containing HECT, UBA and WWE motifs. ARF-BP1 directly binds and ubiquitinates p53; this activity is inhibited by ARF, indicating that ARF-BP1 is a critical mediator of the p53-dependent and p53-independent tumor suppressor functions of ARF. ARF-BP1 can also catalyze the polyubiquitination of Mcl-1, an anti-apoptotic Bcl-2 family member involved in DNA damage-induced apoptosis. Elimination of ARF-BP1 expression by RNA interference stabilized Mcl-1 protein, resulting in an attenuation of apoptosis induced by DNA-damage agents.
• Synonyms: ARF-BP1; HECTH9; HSPC272; Ib772; LASU1; MULE; URE-B1; UREB1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis