RAP80 (UIMC1) Rabbit Polyclonal Antibody

CAT#: TA306471

Rabbit Polyclonal RAP80 Antibody

Product Images

Western blot analysis of RAP80 in 293 cell lysate in (A) the absence and (B) presence of blocking peptide with RAP80 antibody at 1 ug/ml.

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  Immunohistochemistry of RAP80 in human spleen tissue with RAP80 antibody at 2.5 ug/ml.

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  Immunofluorescence of RAP80 in human spleen tissue with RAP80 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 ug/mL, ICC: 2.5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: RAP80 antibody was raised against a 17 amino acid peptide from near the amino terminus of human RAP80.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: ubiquitin interaction motif containing 1
• Database Link:
  NP_057374
  Entrez Gene 20184 MouseEntrez Gene 51720 Human
  UniProt ID Q96RL1
• Background: RAP80 was initially identified as zinc-finger containing nuclear protein that is highly expressed in testis and interacts with the retinoid-related testis-associated receptor (RTR). Later experiments revealed that RAP80 is recruited by the Coiled-coil domain 98 (CCDC98) protein to the breast cancer-1 protein BRCA1, allowing the formation of BRCA1 foci in response to DNA damage caused by ionizing radiation. Both RAP80 and CCDC98 are required for DNA damage resistance, G2-M checkpoint control, and DNA repair. Cells depleted of either RAP80 or CCDC98 exhibited increased sensitivity to ionizing radiation, although not as much as in BRCA1-depleted cells, suggesting that RAP80 and CCDC98 control only part of the DNA damage response role of BRCA1. At least four isoforms of RAP80 are known to exist.
• Synonyms: RAP80; X2HRIP110

Reference Data

• Protein Families: Druggable Genome, Transcription Factors