CNOT4 Rabbit Polyclonal Antibody

CAT#: TA306651

Rabbit Polyclonal CNOT4 Antibody

Product Images

Western blot analysis of CNOT4 in A549 lysate with CNOT4 antibody at (A) 1 and (B) 2 ug/ml.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1 - 2 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: CNOT4 antibody was raised against a 19 amino acid peptide near the amino terminus of the human CNOT4.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: CCR4-NOT transcription complex subunit 4
• Database Link:
  NP_001008226
  Entrez Gene 53621 MouseEntrez Gene 312227 RatEntrez Gene 4850 Human
  UniProt ID O95628
• Background: CNOT4 is a component of the CCR4-NOT transcription complex, a complex that is implicated in the repression of RNA polymerase II transcription. In the CCR4-NOT complex, CNOT4 acts as an E3 ubiquitin-protein ligase and interacts with a subset of E2 ubiquitin-conjugating enzymes through a unique C4C4 RING domain. This E3 ligase activity was shown to be dependent on the selective and specific interaction with the ubiquitin conjugating enzyme UbcH5B. In yeast, mutations in CNOT4 that prevented its interaction with the UbcH5B homolog UBC4 caused increased sensitivity to hydroxyurea, heat shock, and hygromycin B, suggesting that CNOT4 and UbcH5B are involved in stress response in vivo. Multiple isoforms of CNOT4 are known to exist.
• Synonyms: CLONE243; NOT4; NOT4H

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: RNA degradation